Based on a laboratory technique called Thin Layer Chromatography, The Separation Kit is the most powerful evolution in substance identification to date. It’s designed to separate mixed samples BEFORE testing them with Spot Test Kits. Have a sample that contains three different substances? No problem. Now it’s possible to separate them and identify each one independently and accurately – and we’ve somehow managed to make it so easy that anyone can do it.

Not one person involved in the beginning of this project had any idea how powerful of a tool this would turn out to be – it is without a doubt the highest level of accuracy attainable without $30,000 worth of delicate equipment. Some substances are even detectable down to less than 10 micrograms. It’s taken us over three years to develop and perfect this technique – but, at least from where we’re standing, our efforts have been more than worthwhile. Use it often, and use it well.



How do I interpret my results?

When you use the SPOTTING LIGHT to locate and circle the spots/streaks on the TESTING CARD, you’re identifying the location of your separated samples – they will be above the “X MARKS” on the TESTING CARD.

There may be one spot – this indicates a pure substance. You would then use your reagent of choice (Marquis, Ehrlich, etc.) to test and identify that substance.

There may be multiple spots above the same “X MARK” – this most likely indicates that your substance is impure. The reason can be because of deliberate adulteration (cutting) or mistakes in the synthesis/extraction of your substance, leading to the impurity. You can, of course, test these multiple spots to determine the impurity. In either case, you should discard the substance with very few exceptions

There are a small number of substances that will show two spots in pure form, an example being4-AcO-DMT and other 4-AcO substances. These separate into two spots, one spot being the substance itself, and another spot further up on the TESTING CARD. In the case of the substances mentioned above, the second spot is fumaric acid that is used to crystallize the substance, which is harmless. For other exceptions to this rule, please check the SEPARATION TEST KIT FAQ at

If you find that you have a pure substance, it is best to confirm this conclusion by following up with one or more additional tests with different reagents (Marquis, Ehrlich, etc.) using the SEPARATION TEST KIT.

Why are my spots abnormally large?

This happens if you used too much of your sample when dissolving it or placing it on the testing CARD. In this case, you should re-start the SEPARATION TEST KIT process from the beginning. Another option, if necessary, is to dilute your sample by adding more of the TESTINGLIQUID to your original sample in the SMALL VIAL and restarting the SEPARATION TEST KIT process from STEP TWO.

Why don’t I have any (or very small) spots?

The spots should be between 2mm (1/16th of an inch) and 5mm (3/16th of an inch) in diameter for best results. The appearance of no spots or small spots happens if you used too little of your sample when dissolving it in STEP ONE. You’ll need to re-start the SEPARATION TEST KITprocess with a larger sample. If you don’t have any more of your sample, then it is possible to open the SMALL VIAL containing your original sample and allow the testing liquid to evaporate until there is only a little bit left, and then use that to run the test again. If you still don’t see any spots, this means your sample most likely does not contain any active substances – an example being an ecstasy pill that contains nothing but pill binders.

Why do I only see streaks and not spots?

This may happen for three possible reasons:

  • Your TESTING LIQUID is old or was left open and needs to be replaced.
  • Your sample is too concentrated – refer to the question above.
  • Your sample is very impure: This may happen when testing plant material that contains chlorophyll, or naturally extracted samples that haven’t been purified enough. It may also occur because of a very poor synthesis. In either case, it is best to discard the sample.

I can’t interpret the results even though I’ve read everything.

Sometimes impurities, novel substances, or problems with the dilution of samples may make it hard to understand the results. In this case, please take a picture of your plate with the spots and post it on with all the information you can provide for more help. It should also be noted that Mandelin and Simon’s can produce strange results when used with the Skylab.

When putting the testing card in the developing chamber, the testing liquid is rising unevenly – is this a problem?

It is important that the TESTING CARD is placed vertically in the DEVELOPING CHAMBER, leaning slightly back away from the label. It is not a problem if it is rising somewhat unevenly, and it should even out over a couple of minutes. If the TESTING LIQUID is rising very unevenly, however,this can effect the results and make the substance spots from one “X MARK” appear above another “X MARK.” In the worst case scenario, it may even end up on the side of the TESTING CARD. In this, case please repeat the test and remember that the DEVELOPING CHAMBER must be on a level surface.